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plasmid pet28a cas9  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pet28a cas9
    Plasmid Pet28a Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pet28a+cas9+cys/pET28a%2FCas9-Cys+(Plasmid+%2353261)/us12569516-604-28-30
    Average 93 stars, based on 36 article reviews
    plasmid pet28a cas9 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides.
    Article Snippet: Deionized water was purified in-house using an Evoqua Ultra Clear® Glass Panel Systems (Germany) and was used for all experiments. .. Cas9 protein expression and purification Recombinant Cas9 was produced by bacterial expression of a plasmid pET28a/Cas9-Cys containing the human codon-optimized Cas9 nuclease gene with a N-terminal His-tag and a C-terminal cysteine. pET28a/Cas9-Cys was a gift from Hyongbum Kim (Addgene plasmid # 53261 ; http://n2t.net/addgene:53261 ; RRID:Addgene_53261). (65) The plasmid pET28a/Cas9-Cys was transformed into RosettaBL21(DE3)pLysS (Merck Millipore, Germany), as recommended by the manufacturer. ..

    Purification:

    Article Title: Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides.
    Article Snippet: Deionized water was purified in-house using an Evoqua Ultra Clear® Glass Panel Systems (Germany) and was used for all experiments. .. Cas9 protein expression and purification Recombinant Cas9 was produced by bacterial expression of a plasmid pET28a/Cas9-Cys containing the human codon-optimized Cas9 nuclease gene with a N-terminal His-tag and a C-terminal cysteine. pET28a/Cas9-Cys was a gift from Hyongbum Kim (Addgene plasmid # 53261 ; http://n2t.net/addgene:53261 ; RRID:Addgene_53261). (65) The plasmid pET28a/Cas9-Cys was transformed into RosettaBL21(DE3)pLysS (Merck Millipore, Germany), as recommended by the manufacturer. ..

    Recombinant:

    Article Title: Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides.
    Article Snippet: Deionized water was purified in-house using an Evoqua Ultra Clear® Glass Panel Systems (Germany) and was used for all experiments. .. Cas9 protein expression and purification Recombinant Cas9 was produced by bacterial expression of a plasmid pET28a/Cas9-Cys containing the human codon-optimized Cas9 nuclease gene with a N-terminal His-tag and a C-terminal cysteine. pET28a/Cas9-Cys was a gift from Hyongbum Kim (Addgene plasmid # 53261 ; http://n2t.net/addgene:53261 ; RRID:Addgene_53261). (65) The plasmid pET28a/Cas9-Cys was transformed into RosettaBL21(DE3)pLysS (Merck Millipore, Germany), as recommended by the manufacturer. ..

    Produced:

    Article Title: Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides.
    Article Snippet: Deionized water was purified in-house using an Evoqua Ultra Clear® Glass Panel Systems (Germany) and was used for all experiments. .. Cas9 protein expression and purification Recombinant Cas9 was produced by bacterial expression of a plasmid pET28a/Cas9-Cys containing the human codon-optimized Cas9 nuclease gene with a N-terminal His-tag and a C-terminal cysteine. pET28a/Cas9-Cys was a gift from Hyongbum Kim (Addgene plasmid # 53261 ; http://n2t.net/addgene:53261 ; RRID:Addgene_53261). (65) The plasmid pET28a/Cas9-Cys was transformed into RosettaBL21(DE3)pLysS (Merck Millipore, Germany), as recommended by the manufacturer. ..

    Plasmid Preparation:

    Article Title: Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides.
    Article Snippet: Deionized water was purified in-house using an Evoqua Ultra Clear® Glass Panel Systems (Germany) and was used for all experiments. .. Cas9 protein expression and purification Recombinant Cas9 was produced by bacterial expression of a plasmid pET28a/Cas9-Cys containing the human codon-optimized Cas9 nuclease gene with a N-terminal His-tag and a C-terminal cysteine. pET28a/Cas9-Cys was a gift from Hyongbum Kim (Addgene plasmid # 53261 ; http://n2t.net/addgene:53261 ; RRID:Addgene_53261). (65) The plasmid pET28a/Cas9-Cys was transformed into RosettaBL21(DE3)pLysS (Merck Millipore, Germany), as recommended by the manufacturer. ..

    Article Title: The antifungal peptide AnAFP from Aspergillus niger promotes nutrient mobilization through autophagic recycling during asexual development
    Article Snippet: .. Cas9 (fused N-terminally to a 6xHis tag and C-terminally to the nuclear localization sequence KKRKV) was expressed in E. coli CZ4.1, using the plasmid pET28a/Cas9-Cys (Addgene #53261). .. The donor DNAs for BBA22.6, STS4.10, STS9.2 and JW3.3 were assembled via Gibson cloning and the DNAs for STS19.3, LV1.1 and LV2.3 were generated via the modular cloning kit from Addgene ( ).

    Transformation Assay:

    Article Title: Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides.
    Article Snippet: Deionized water was purified in-house using an Evoqua Ultra Clear® Glass Panel Systems (Germany) and was used for all experiments. .. Cas9 protein expression and purification Recombinant Cas9 was produced by bacterial expression of a plasmid pET28a/Cas9-Cys containing the human codon-optimized Cas9 nuclease gene with a N-terminal His-tag and a C-terminal cysteine. pET28a/Cas9-Cys was a gift from Hyongbum Kim (Addgene plasmid # 53261 ; http://n2t.net/addgene:53261 ; RRID:Addgene_53261). (65) The plasmid pET28a/Cas9-Cys was transformed into RosettaBL21(DE3)pLysS (Merck Millipore, Germany), as recommended by the manufacturer. ..

    Sequencing:

    Article Title: The antifungal peptide AnAFP from Aspergillus niger promotes nutrient mobilization through autophagic recycling during asexual development
    Article Snippet: .. Cas9 (fused N-terminally to a 6xHis tag and C-terminally to the nuclear localization sequence KKRKV) was expressed in E. coli CZ4.1, using the plasmid pET28a/Cas9-Cys (Addgene #53261). .. The donor DNAs for BBA22.6, STS4.10, STS9.2 and JW3.3 were assembled via Gibson cloning and the DNAs for STS19.3, LV1.1 and LV2.3 were generated via the modular cloning kit from Addgene ( ).



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